ATP1A1 anticorps

N° du produit ABIN215644

Cet anticorps Souris Monoclonal détecte spécifiquement ATP1A1 dans WB, IHC et IP. Il présente une réactivité envers Humain, Rat, Souris, Porc, Chien, Singe et Xenopus laevis.

Aperçu rapide pour ATP1A1 anticorps (ABIN215644)

Antigène: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivité: Humain, Rat, Souris, Porc, Chien, Singe, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ATP1A1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Détail du produit anti-ATP1A1 anticorps

Specificité: Recognizes rabbit Na+/K+ ATPase alpha-1 subunit. Species cross-reactivity: Human, rabbit, monkey, rat, mouse, lamb, canine, porcine and Xenopus.

Purification: Protein G purified

Immunogène: Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: IHC, IP, WB (0.05 - 0.1 μg/mL)

Usage: Western Blot: 0.05-0.1 μg/mL detected the a-1 subunit in 20 μg of rat brain microsomal preparations using chemiluminescence. To reduce background, wash the blot with 0.05 % Tween-20 in PBS for 15 minutes.Immunohistochemistry: 1-10 μg/mL on ice-cold methanol-fixed tissue.Immunoprecipitation: 2 μg immunoprecipitated the a-1 subunit from rat brain microsomal preparations.Optimal dilutions to be determined by the researcher.

Commentaires:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 0.1M Tris-Glycine,  pH 7.4, 0.15M sodium chloride, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur ATP1A1

Antigène: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Autre désignation: ATP1A1

Sujet: Name/Gene ID: ATP1A1
Subfamily: ATPase - P type, type IIC
Family: Transporter

Synonyms: ATP1A1, Na+, K+ ATPase alpha subunit, Na,K-ATPase alpha-1 subunit, Na+/K+ ATPase 1, Sodium pump 1, Sodium pump subunit alpha-1

ID gène: 476

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process